Evaluation of the Effects of High Fat Diet Feeding on Experimental Infection by Trypanosoma cruzi
Name: ELISA LIZ BELLI CASSA DOMINGUES
Publication date: 24/07/2017
Advisor:
Name |
Role |
|---|---|
| FABIANE MATOS DOS SANTOS | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| BLIMA FUX | External Examiner * |
| FABIANE MATOS DOS SANTOS | Advisor * |
| FABIANO KENJI HARAGUCHI | Internal Examiner * |
| GUSTAVO ROCHA LEITE | External Alternate * |
| LUCIANE BRESCIANI SALAROLI | Internal Alternate * |
Summary: Chagas disease (DC) is a parasitic disease with the etiological agent Trypanosoma cruzi. Chagasic heart disease is an important infection of T. cruzi infection and can manifest severe cases of heart failure with evolution to sudden death. In addition to the parasitic etiology, others are also the causes that can lead to the development of cardiovascular diseases with signs of heart failure, such as consumption of the hyperlipid diet and the inflammatory process corroborated by dyslipidemias. Thus, the objective of the present study was to evaluate the effects of the hyperlipidic diet on T. cruzi experimental infection. Body weight and food intake were measured weekly. The parasitemia was monitored daily in the acute phase of infection and biochemical parameters were evaluated monthly for a period of 90 days of infection. For histopathological evaluations, fragments of adipose tissue and cardiac muscle tissue were collected after euthanasia of the animals at 90 days of infection. The experimental procedures were approved by the Ethics Committee on the Use of Animals of the Federal University of Espírito Santo (protocol no 043/2015). The hyperlipidic diet led to a reduction of parasitemia in the acute phase of infection (p <0.05), elevation of body weight mainly in the uninfected group and fed a hyperlipid diet, reduction in mean dietary intake and elevation of serum total cholesterol levels Infection (p <0.05). Increased adipose tissue hypertrophy (p <0.001) in uninfected animals fed a hyperlipidic diet, and an increase in inflammatory cells in the cardiac muscle tissue (p <0.001) following the elevated serum levels of TNF-alpha cytokine in animals Infected individuals who consumed a hyperlipidic diet. These results demonstrate that the high lipid consumption resulted in hypertrophy of adipose tissue and maintenance of the cardiac inflammatory process.
Keywords: Trypanosama cruzi, High-fat diet, Chagas heart disease
