Identification of Biochemical Changes in the Serum of Individuals Undergoing Roux-en-Y Gastric Bypass Surgery by Fourier
Transforminfrared vibrational spectroscopy
Name: AMANDA MOTTA DE BORTOLI
Publication date: 11/06/2024
Examining board:
Name |
Role |
|---|---|
| FABIANO KENJI HARAGUCHI | Presidente |
| LÍVIA CARLA DE MELO RODRIGUES | Examinador Interno |
| LUCIENE CRISTINA GASTALHO CAMPOS | Examinador Externo |
| VALERIO GARRONE BARAUNA | Coorientador |
Summary: Bariatric surgery, especially Roux-en-Y gastric bypass (RYGB), is the most
indicated treatment for severe obesity, promoting significant biochemical
alterations. Fourier transform infrared vibrational spectroscopy (FTIR) has
been applied in biological analyses with the potential to detect such changes.
This study investigated the use of FTIR for identifying biochemical alterations in
the serum of individuals undergoing RYGB. An observational and descriptive study
was conducted with patients from the Bariatric and Metabolic Surgery Program
of a university hospital, before (T0) and approximately two months after RYGB
(T1). Biochemical parameters, anthropometric data, and body composition were
evaluated. FTIR spectra were recorded using ALPHA II equipment (Bruker,
Germany), pre-processed, and subjected to Principal Component Analysis
(PCA) and Partial Least Squares Discriminant Analysis (PLS-DA) (MATLAB
2023b). Data normality was tested using the Kolmogorov-Smirnov test, followed
by paired T-test or Wilcoxon test. Spearman correlation analysis of spectral
information with biochemical parameters was performed. A significance level
of p <0.05 was adopted for all tests (GraphPad Prism 8 software). Twenty-nine
individuals (86.2% female) with a mean age of 41.2 ± 7.8 years were
evaluated. Anthropometric and body composition variables significantly decreased (p
<0.001). Serum concentrations of albumin, transthyretin, C-reactive protein,
alkaline phosphatase, glucose, triglycerides, total cholesterol, and LDL
cholesterol also showed significant reductions (p <0.05). PCA demonstrated a
trend of separation at T0 and T1 generated by biochemical alterations.
Different significant correlations between biochemical parameters and spectral regions
were identified at T0 and T1; however, only serum triglyceride concentration
at both time points (p <0.05) correlated significantly with the spectral region
characteristic of lipids (1796 - 1685 cm -1 ). The results suggest that FTIR
exhibits sensitivity to identify alterations related to the lipid profile of
individuals undergoing surgical treatment for severe obesity.
